A simple, specific, accurate and precise reversed phase high pressure liquid chromatography (RP-HPLC) method has been developed for the simultaneous estimation of tramadol hydrochloride (TRA) and dicyclomine (DCY) in bulk and tablet dosage form. Chromatography was carried on Phenomex Gemini C column (4.6 x 250 mm, 5u particle size) using mixture of methanol, acetonitrile and 0.1% triethylamine (TEA) pH 3.0 (adjusted with orthophophoric acid) in the ratio of 35:5:60 (v/v/v) respectively as a mobile phase. The flow rate 1.0mL min-1 and detection wavelength 275 nm was selected for the present study. The retention time of tramadol hydrochloride and dicyclomine were 4.13 min and 9.06 min respectively. The linear regression analysis data for the calibration plots showed a good linear relationship over the concentration range of 50-300 μg mL-1 for tramadol hydrochloride and 10-60 μg mL-1 for dicyclomine. The proposed method was validated with respect to linearity, precision, accuracy, specificity and robustness. The proposed method was found to be simple, rapid, accurate, precise and reproducible. Thus it can be applied for routine quality control analysis of tramadol hydrochloride and dicyclomine in tablet dosage form.

In the present study an isocratic reverse phase-high performance liquid chromatography (RP-HPLC) method was developed and validated for simultaneous estimation of dicyclomine hydrochloride (DCY) and famotidine (FAM) in bulk and pharmaceutical dosage forms. The separation was achieved on a Phenomex Gemini C column (4.6 x 250 mm, 5μ particle size) using a mixture of methanol and 0.1% triethylamine (TEA) pH 3.0 (adjusted with orthophosphoric acid) in the ratio of 40:60 (v/v) as a mobile phase. The flow rate was 1.0 mL min-1, column temperature was kept at 28 0 C and detection was monitored at 270 nm. The retention time of dicyclomine hydrochloride and famotidine was found to be 10.72 and 2.72 min respectively. Recovery studies were satisfactory and the correlation coefficient for FAM and DCY were 0.9947 and 0.9935 respectively, indicates linearity of the methods within the limits. The developed method can be used for routine quality control simultaneous analysis of DCY and FAM in tablet dosage form without any interference of excipients.

Nos últimos anos, o estresse oxidativo e radicais livres têm sido implicados na cicatrização. Abelmoschus manihot (L.) Medik., Malvaceae e Wrightia tinctoria R. Br., Apocynaceae, plantas utilizadas na medicina Ayurveda, possuem propriedades antiinflamatórias e antimicrobianas. O presente estudo foi realizado para avaliar o potencial dos extratos de éter de petróleo e metanólico na cicatrização de feridas em ratos Wistar. Os ratos foram divididos em seis grupos com seis animais cada. O grupo 1 foi utilizado como controle, o grupo 2 recebeu a droga padrão e os outros quatro grupos foram tratados com duas doses diferentes de cada um dos extratos de A. manihot e W. tinctoria. Os parâmetros de cicatrização foram avaliados através da incisão feridas em ratos tratados com extrato, padrões e controles. Ambas as doses dos extratos de éter de petróleo e metanólico aumentaram significativamente força de ruptura da ferida quando comparados ao grupo controle.

In recent years, oxidative stress and free radicals have been implicated in impaired wound healing. Abelmoschus manihot (L.) Medik., Malvaceae, and Wrightia tinctoria R. Br., Apocynaceae, plants widely used in Ayurveda, possesses anti-inflammatory and antimicrobial properties. The present study was undertaken to assess the potential of petroleum ether and methanolic extracts in wound healing in Wistar albino rats. The rats were divided into six groups of six animals each. Group 1 is normal wounded control, group 2 received standard drug and the other four groups were treated with two different doses each of petroleum ether and methanolic extract of A. manihot and W. tinctoria. The wound healing parameters were evaluated by using incision wounds in extract-treated rats, standard and controls. Both the doses of petroleum ether and methanolic extract significantly increased wound breaking strength when compared with the control group.